Tailed Primer Base Excision Sequence Scanning (TP-BESS) for Detection of Single Nucleotide Polymorphisms (SNPs)

Topics:
Pharmeceuticals
Tags:
Canada,
Detection
Source:
National Research Council of Canada

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Overview: Base excision sequence scanning (BESS) is used to detect and localize point mutations in mammalian genes. The cost of BESS can be significant in large-scale projects, however, due to the requirement for end-labeling of one of the two PCR primers. This is especially true when a fluorescent label is used for detection. The paper describes a TP-BESS procedure where one fluorescence-labeled primer which is used as a universal label for all the PCR reactions in BESS analysis. The universal label makes BESS financially more feasible for large-scale detection of single nucleotide polymorphisms (SNPs).

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Format: PDF | Size: 46KB | Date: Mar 2001 | Pages: 6

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